eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-03-25 14 1 415 419 10.13005/bbra/2460 22999 article Biotin Axotroph Mutants of Native Corynebacterium Glutamicum for the Fermentative Production of L-Glutamic Acid V. Krishnarjuna Reddy 1 U. Keerthi 1 A. V. N. Swamy 2 Dowlathabad Muralidhara Rao 1 Department of Biotechnology, Sri Krishnadevaraya University, Ananthapuramu, India. Department of Chemical Engineering, Jawaharlal Nehru Technological University Ananthapuram, Ananthapuramu, India.

The present paper deals with isolation of C.glutamicum strain and strain improvement of C.glutamicum by mutation studies for the production of L-Glutamic acid. Native isolate of C.glutamicumwas manipulated to excrete L-glutamate. The modified protocol was used in this paper to trigger L-glutamate excretion. The important feature of this protocol is to achieve a biotin concentration that is low enough to activate glutamate excretion but high adequate to still allow sufficient growth of the cells. To achieve this appropriate biotin concentration, a pre cultivation step is required in order to deplete the cells of biotin. Later Physical Mutation was induced on Biotin depleted C.glutamicum strain.

 https://www.biotech-asia.org/vol14no1/biotin-axotroph-mutants-of-native-corynebacterium-glutamicum-for-the-fermentative-production-of-l-glutamic-acid/

Biotin;Corynebacterium glutamicum: Fermentation L-Glutamic acid;