eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-09-25 13 3 1409 1413 10.13005/bbra/2283 15709 article Asep A. Prihanto 1 Abdul A. Jaziri 1 Ima Y. Perwira 2 Department of Fishery and Marine Science, Faculty of Fisheries and Marine Science, Brawijaya University, Veteran street, Malang, 651245, East Java Indonesia. Department of Aquatic Resources Management, Faculty of Marine Science and Fisheries, Udayana University, Bali - Indonesia. Neutral protease producing bacterium Bacillus substilis UBT7 strain isolated from Terasi, an Indonesian fermented fish grew in shake flask and fermenter, and produced protease at 37 °C, pH 7 for 24 hour. After three purification steps, the enzyme was successfully purified. This neutral protease had an apparent molecular mass of 32 kDa as estimated by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme was active optimum on 50 °C. This indicated that the enzyme was slightly thermozyme. The stability was decreased on 40 °C. The enzyme was optimum in pH 7. The enzyme was somehow enhanced with Fe²⁺ and K²⁺ metal ionsbut not by the addition of Ni²⁺. https://www.biotech-asia.org/vol13no3/purification-and-characterization-of-neutral-protease-from-bacillus-substilis-ubt7-isolated-from-terasi-indonesian-fermented-fish/ Terasi; Bacillus substilis UBT7; neutral protease; fermented fish; Indonesia