eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-09-25 13 3 1333 1340 10.13005/bbra/2274 15656 article Trianik Widyaningrum Indro Prastowo 1,4 Masreza Parahadi 3 Angga Dwi Prasetyo 3 Department of Biology Education, Faculty of Teacher Training and Education, Ahmad Dahlan University, Kampus III, Jl. Prof. Dr.Soepomo, Janturan, Yogyakarta 55164, Indonesia. Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Gedung Biologi, Jl. Veteran, Malang 65145, Indonesia. Department of Postgraduate Program in Biotechnology, Postgraduate School, Gadjah Mada University, Jl. Teknika Utara, Barek Yogyakarta 55281, Indonesia. Nitidipuran Life Science Studio, Jl. Nitidipuran 26, Ngupasan, Gondomanan, Yogyakarta 55122, Indonesia. The efforts to produce bioethanol using non-crop raw material are recently developed. Sargassum crassifolium is a promising raw material for biofuel production since containing a high concentration of polysaccharides. Pre-treatment of seaweed at working pressure of 15 Psi, temperature of 121 ^oC and sulphuric acid concentration of 0.2 M increased the reducing sugars concentration up to 26.68 g/l. The application of cellulase (0.5 g/ml) helped increasing the reducing sugars concentration up to 2.56 times (increasing up to 68.32 g/l). Fermentation using a naturally β-glucosidase producing yeast, Saccharomyces cereviceae (JCM 3012) (0.15 g/ml), produced the final bioethanol conversion up to 90.99%. The activity of β-glucosidase increased up to 20.39 U/ml during fermentation that assisted the degradation of seaweed. https://www.biotech-asia.org/vol13no3/production-of-bioethanol-from-the-hydrolysate-of-brown-seaweed-sargassum-crassifolium-using-a-naturally-%ce%b2-glucosidase-producing-yeast-saccharomyces-cereviceae-jcm-3012/ Bioethanol; cellulase; seaweed Sargassum crassifolium; Saccharomyces cereviceae JCM 3012; β-glucosidase