eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-06-25 13 2 1253 1258 10.13005/bbra/2160 14290 article Davood Moghadamnia 1, 2 Mokhtar Mokhtari 3 Saeed Khatamsaz 3 Department of Biology, Fars Science and Research Branch ,Islamic Azad University, Fars, Iran. Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran. Department of Biology ,Kazerun Branch, Islamic Azad University, Kazerun, Iran. exposure to thioacetamide causes hepatotoxicity and hepatocellular carcinoma in human, while fish oil Omega-3 supplement has anti-inflammatory effects. In this study, the protective effect of Fish oil Omega-3 supplement against  liver dysfunction induced by thioacetamide in rat is investigated.42 male rats were divided into 6 groups of seven. The control group, The sham 1 group receiving 0.4ml/kg olive oil as the solvent of Fish oil Omega-3 supplement, the sham2 group inter peritonealy receiving a dose of 150 mg/kg thioacetamide at the end of the experiment, the experimental groups 1, 2 and 3 orally receiving a daily dose of 100, 200 and 300 mg/kg Fish oil Omega-3 supplements respectively for 3 month followed by an inter peritoneal dose of 150 mg/kg thioacetamide at the end of the experiment. The serum levels of SGOT, SGPT, ALP and bilirubin were measured. Following hematoxylin-eosin staining, liver tissue samples were pathologically studied.The mean concentration of SGPT and bilirubin showed a significant decrease in the experimental group 1 compared to the group receiving thioacetamide. The mean levels of ALP  and  SGOT showed no significant changes in the experimental groups 1, 2, and 3. Finally, the tissue changes in all three experimental groups improved compared to the group receiving thioacetamide, and these effects were dose-dependent (p≤.05). The results of this study indicate that Fish oil Omega-3 supplement has a protective effect on liver dysfunction induced by thioacetamide in rat. https://www.biotech-asia.org/vol13no2/protective-effects-of-fish-oil-omega-3-supplement-on-thioacetamide-induced-liver-dysfunction-in-male-rats/ Fish Oil Omega-3 Supplement;Thioacetamide;Liver Dysfunction;Male Rat