eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-06-25 13 2 821 826 10.13005/bbra/2102 14013 article Shahin Moradi 1 Namdar Yousofvand 1 Department of Biology, Faculty of Science, University of razi, Kermanshah, Iran Peganum harmala and piper Longum medicinal herbs are used in traditional medicine to treat various diseases and to relieve tragic processes. The chemical composition of these two compounds is their analgesic agent. The purpose of this study was the Peganum harmala and piper Longum rubbing oil effect on pain in male mice by using formalin test. In this study, male mice were divided into three groups: control group, Peganum harmala rubbing oil group, piper Longum rubbing oil group. All groups were taken formalin test and data were analyzed by using Graphpad prism statistical software. In rubbing groups, Peganum harmala rubbing oil and the piper Longum rubbing oil, there was a significant difference compared with the control group (p<0.05). It seems that the reason of these two plants analgesic should be sought in their chemical composition. The chemical compositions of these two plants have analgesic effects. https://www.biotech-asia.org/vol13no2/peganum-harmala-and-piper-longum-plant-rubbing-oil-effect-on-pain-in-small-male-mice/ Pain; Peganum harmala oil; Piper Longum oil; Formalin test; Mice