eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-31 13 1 505 514 10.13005/bbra/2062 7625 article O. A. Konovalova 1 N. V. Kalacheva 1 G. V. Cherepnev 1 M. V. Trushin 1 Kazan Federal University, Kremlevskaya str., 18, 420008, Kazan, Russia. A comparative study of the physical adsorption of RNAse A and RNAse Bacillus pumilis onto a negatively charged surface of mica, a hydrophobic surface of pyrolytic graphite and a surface of lipid layers of dipalmitoylphosphatidylcholine (DPPC) was performed by atomic force microscopy. It was found that microbial RNAse, unlike RNAse A, 1) is adsorbed onto the negatively charged surface of mica in the form of monomers and dimers; 2) exhibits enhanced tropism to the hydrophobic surface of pyrolytic graphite; 3) modifies morphotopography and thickness of the lipid bilayer of DPPC. The detected surface-dependent differences in adsorption of RNAses are consistent with the features of their structure and cytotoxic properties. https://www.biotech-asia.org/vol13no1/surface-dependent-differences-in-the-adsorption-of-pancreatic-and-microbial-ribonucleases-visualized-by-atomic-force-microscopy/ microscopy; morphotopography ; adsorption of pancreatic