eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-31 13 1 95 101 10.13005/bbra/2009 7652 article Hussam H. Arafat 1 Mohammed H. El-Sayed 2 Askora A. 3 Department of Biology, Faculty of Science and Arts, Northern Border University, Rafha, KSA. Department of Botany, Faculty of Science, Zagazig University, Zagazig City- 44519, Egypt. Department of Botany and Microbiology, Faculty of Science, Minia University, Minia City- 61519, Egypt. Antibacterial activities of Ag-NPs have received much attention due to their effective killing and cost-effectiveness. Biosynthesis is an attractive and eco-friendly method to produce silver nanoparticles (Ag-NPs). Ag-NPs are considered a promising tool to overcome the emergence of multi-drug resistant bacteria. In this study, bio-synthesis of Ag-NPs was attempted using plant extracts of peppermint. Characterization of Ag-NPs was achieved by UV-visible spectrophotometer and TEM. Monodispersed Ag-NPs were obtained with different sizes ranged from 6 to 88 nm. Antibacterial activities of Ag-NPs against pathogenic bacteria were evaluated using disc diffusion method.Our results indicated that Gram positive bacteria were more susceptible than Gram negative. The current study offers a cost-effective and eco-friendly method for biosynthesis of potent bactericidal Ag-NPs and their use against human pathogenic bacteria. https://www.biotech-asia.org/vol13no1/silver-nanoweapons-a-novel-tool-against-multidrug-resistant-bacteria/ silver nanoparticles; pathogenic bacteria; green synthesis; silver nanoweapons