eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-03-31 13 1 37 42 10.13005/bbra/2000 7033 article Ehsan Barati 1 Sara Soleimani Asl 2 Seyed Ali pourbakhsh 1 Mahmoud Jamshidian 1 Siamak Shahidi 2 Department of Microbiology,Science and Research Branch,Islamic Azad University,Tehran,Iran Department of Neurophysiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan 65178-3-8736, Iran Alzheimer’s disease (AD) is a neuro degenerative disorder and most common form of dementia that leads to memory impairment. In the present study we have examined the investigating effect of Borago officinale on hipocampal TNF-α protein in the Beta Amyloid peptid induced of inflammation in the rat. Wistar male rats received intrahippocampal (IHP) injection of the Aβ(25–35) and borage extract orally (100 mg/kg).inflamation was confirmed by pathologist.in order to measure the expression of the TNF-α gene , RT- PCR method and for extraction of the TNF-α protein western bloting method was used. results showed that using Borage lead to reduce TNF-α gene and protein. This data suggests that borage could improve the reduction of inflammation factors such as TNF-α and borage consumption may lead to an improvement of AD-induced cognitive dysfunction. https://www.biotech-asia.org/vol13no1/effect-of-borage-on-hipocampal-tnf-%ce%b1-protein-and-gene-in-the-amyloid-%ce%b2-peptide25-35-induced-of-alzheimer-model-in-rat/ TNF-α; Alzheimer; RT-PCR; Western Blot; Amyloid β-Peptide