eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2015-12-25 12 2 1339 1344 2434 article Maryam Sadat Abtahi 1* Hossein Maghsoudi 1 Boshra Hatef 2 Parisa Marashi 3 Mahnesa Vassaf 3 Department of Biotechnology Engineering the agricultural orientation, Payame Noor University, po box, 19395-3697, Tehran, IRAN Department of Biotechnology, Payame Noor University, po box, 19395-3697, Tehran, IRAN Professor assistant of Neuroscience Research Center of Baqiyatallah University of Medical Science, North Artesh Highway, Second Avenue, Tehran, IRAN Department of Biotechnology Engineering the agricultural orientation, Payame Noor University, po box, 19395-3697, Tehran, IRAN The Trachyspermum ammi plant has anti-fungal properties. The aim of this study is to examine the anti-fungal  effects of this plant on the pro-inflammatory  bio-markers, which are important in the course of fungal diseases. At first the ethanol extract of the Trachyspermum ammi plant was prepared from the Iranian biological resource center. We used monocyte/macrophage cells as samples because they were similar to the cells with fungal infection. Then THP-1 monocyte/macrophages (5×10⁵ cells/well) were incubated at a humidity of 90%,  5% CO2, 37˚C for 72 h with control media alone or Trachyspermum ammi at concentrations of 10 μg/ml. One set of cells was activated for 1 h with mannose (3 mg/ml) for both reverse-transcriptase Polymerase chain reaction and real-time Polymerase chain reaction analysis of Tumor necrosis factor alpha (TNF-α), Interleukin-18 (IL-18) expression. the activated cells by mannose with  a plant extract  redused the Interleukin-18 and Tumor necrosis factor alpha expression as  activated cell by mannose with  fluconazole (p-value=0.91 for IL-18, p-valu=0.25 for TNF-α). Based on the PCR and realtime methode to analysis of gene expression,  Trachyspermum ammi plant such as the flavonoids has noticeable anti-fungal properties. https://www.biotech-asia.org/vol12no2/antifungal-effect-of-flavonoid-extract-of-trachyspermum-ammi-plant-on-the-gene-expression-of-pro-inflammatory-cytokines-such-as-il-18-and-tnf-%ce%b1-in-articular-thp-1-monocytemacrophages-cells/ Trachyspermum ammi; THP-1 Monocyte/Macrophage; Fungal diseases