eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2015-08-15 12 2 1075 1079 2743 article Woo Tae Park 1 Sang Un Park 1 Sun Kyung Yeo 1 Md Romij Uddin 2 Haeng Hoon Kim 3 Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Korea Department of Agronomy, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Department of Well-being Resources, Sunchon National University, 413 Jungangno, Suncheon, Jeollanam-do, 540-742, Korea We developed a rapid protocol for efficient shoot organogenesis and plant regeneration from stem node cultures of Polygonummultiflorum. Stem node explants were cultured with varying concentrations of cytokinins, then with 0.5 mg/l of 6-benzylaminopurine (BAP) supplemented with various concentrations of different auxins, and regeneration capacity and growth of shoots were assessed. Among the cytokinins, BAP induced shoot initiation with the highest efficiency, whereas zeatin showed higher efficacy for stimulation of shoot growth after 6 weeks in culture. BAP with indole-3-acetic acid (IAA) was highly effective in stimulating shoot initiation. Among the treatment combinations, 0.5 mg/l BAP with 1.0 mg/l IAA proved optimal yielding the highest number of shoots (3.1 per explant). This system can produce approximately 4 plantlets in vitro from one stem node explant culture of P. multiflorum, and the system can be used at any time of the year. https://www.biotech-asia.org/vol12no2/an-efficient-protocol-for-shoot-organogenesis-and-plant-regeneration-of-polygonummultiflorum/ plant regeneration; Polygonummultiflorum; shoot organogenesis