eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2015-04-28 12 1 379 385 5698 article Mohammad S. AL-Harbi 1 Tahani A.Al-Sofiani 1 Reham Z. Hamza 2 Department of Biology, Faculty of Science, Taif University, Taif 888, Saudi Arabia Department of Zoology, Faculty of Science , Zagazig University , Zagazig 44519, Egypt. Paracetamol (APAP-Acetaminophen) has been used extensively as antipyretic drug. The purpose of this study was to evaluate the ameliorative role of silymarin or/and Nigella sativa extract against APAP-induced hematotoxicity in male mice at the hematological and immunological levels. The mice were divided into seven groups (10/ group). The first group was served as control. While, the second group was treated with dose of APAP. The third and fourth groups were treated with silymarin alone and Nigella sativa extract alone respectively, the fifth and sixth groups were treated with combination of APAP with silymarin and APAP with Nigella sativa extract respectively. The seventh group was treated with a combination of both ameliorative compounds (Silymarin and Nigella sativa extract) with APAP and all animals were treated for a period of 30 days. Exposure to APAP at the treated dose to mice led to an alteration of hematological and immunological parameters, increase W.B.Cs and TNF-, decreased R.B.Cs and Hb levels in APAP treated group. Administration of silymarin or/and Nigella sativa extract to APAP-treated mice alleviate the hematotoxicity of APAP, and this appeared clearly by biochemical improvement of hematological picture. But, the alleviation is more pronounced with the both antioxidants. Thus, the pronounce effect of Silymarin and Nigella sativa extract is most effective in reducing the hematotoxicity and immunotoxicity induced by APAP and improving immunological capacities of male mice. https://www.biotech-asia.org/vol12no1/ameliorative-roles-of-silymarin-and-nigella-sativa-on-hematological-parameters-and-immunological-capacities-of-male-mice-affected-by-paracetamol/ Paracetamol; Silymarin; Nigella sativa extract; Hematological parameters; Immunological parameters