eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-06-20 12 Spl.Edn.2 369 373 10.13005/bbra/2212 12939 article Uma Sudhakar 1 T.Ramakrishnan 2 A. Rekha 3 H.Tamizhchelvan 4 V. Shankar Ram 5 Kamal Kannadasan 6 Professor & Head, Thaimoogambigai Dental College, Professor, VP, Meenakshi Ammal Dental College, PG Student,Thaimoogambigai Dental College, Professor, Oral Pathology, Sri Ramachandra Dental College, Reader, Thaimoogambigai Dental College, Professor, Oral Surgery ,Thaimoogambigai Dental College, Chronic Periodontitis, an inflammatory disease caused by oral bacteria stimulates the host cells,neutrophils which release Reactive Oxygen Species(ROS) as a part of immune response. Excess ROS is one of the pathological features in the periodontal lesion.Recently, Reactive Oxygen Metabolites(ROM) were recognized as a useful measure of blood ROS .The aim of this study is to estimate the prevalence of ROM in Plasma, saliva and Gingival crevicular fluid in Chronic Periodontitis, chronic gingivitis and healthy controls. The study population consisted of 45 subjects belonging to both the sexes were randomly selected . Subjects were divided into three groups , Healthy periodontium ( HP)(Group I) and Chronic Gingivitis (CG)(Group II), Chronic Periodontitis(CP) ( Group III). GCF, saliva and plasma were collected in all the three groups to estimate the Reactive oxygen metabolite levels. ROM levels in plasma concentration were almost the same in all the groups [ pvalue 0.13]. The values obtained in saliva and GCF were significantly higher in Chronic Periodontitis group compared to Chronic Gingivitis and Healthy Periodontium.[ pvalue < 0.001]. The results of our study suggested that a significant oxidative stress may occur in Periodontitis. The findings also suggest that it might play an important role in the pathology of Periodontitis and the associated tissue damage. https://www.biotech-asia.org/vol12_nospl_edn2/prevalence-of-reactive-oxygen-metabolite-levels-in-plasma-gcf-and-saliva-in-chronic-periodontitis-chronic-gingivitis-and-healthy-periodontium-a-biochemical-study-2/ ROM; plasma; saliva ; GCF: Chronic Periodontitis