Nicarbazin is an effective and wide spread recognized substance for controlling the protozoal diseases with low number of short term adverse reactions. There are some molecular mechanisms proposed for its adverse effect but no one has conducted a research in vivo. Also there is no evidence found which can illustrate the long term adverse effects of the drug. It may hypothesized that Nicarbazin can affect the oxidative situation of cells by lipoprotein lipase activation. This study investigated the relationship between using Nicarbazin or its ingredients and oxidative stress situation by monitoring changes in the oxidative enzymes activity:Catalase, Glutathione peroxidase, Superoxide dismutase and specific biomarkers of oxidation: Dityrosine and Malondialdehyde. The study also investigates Nicarbazin's effects on colony stimulating factors in lung cells. In conclusion it found that Nicarbazin can worsen oxidative stress and increase colony stimulating factors which may have affect on long term adverse reactions of drug.