eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2014-04-28 11 1 6466 article R. Mensudar 1 D. Amudha 2 Department of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospitals, Bharath University, Pallikaranai, Chennai, India Sree Balaji Dental College, Bharath University, India. In day-to-day clinical practice dentists face with many kinds of micro-organisms because of their professions that require intimate contact with their patients. Thus, sterilization and disinfection methods should be implemented meticulously and their effectiveness carries a crucial importance for the physician and the patient's health. Assurance of sterility of instruments and devices can be obtained through the use of one of several tests and these tests must be performed regularly to ensure that the sterilizer is sterilizing all instruments and devices and that these are safe for use on patients. https://www.biotech-asia.org/vol11no1/newer-sterilization-methods/ sterilization; ozone; plasma