eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2013-06-28 10 1 10.13005/bbra/1111 10307 article Zaki M. Eisa 1 Ahmed Agag 2 Mona M. Al-Dawsary 3 Saleh A. Eifan 4 Basheer A. Al-Sum 4 Virology Consultant, King Fahd Hospital, Jazan University of Jazan, Biology Department College of Science and Humanities, Salman bin AbdelAziz University, Department of Botany and Microbiology, College of Science, King Saud University. This study determines the immune status against Toxoplasma gondii in human and animal sera in Jazan district using different techniques. Sera from 124 women, 50 men, 16 infants, randomly were obtained from king Fahd central hospital outpatients' clinics, 30 sheep 31 goat were obtained from animals attending the veterinary clinics for any reasons. Sera were employed for Toxoplasma gondii latex agglutination (LA), Indirec hemagglutination (IHA) & Enzyme-Linked Immunosorbent Assay [ELISA (IgG & IgM)]. The results revealed anti-toxoplasma by LA, IHA & ELISA (IgG & IgM) as follow 28.22%, 33.87%, 41.9 and %5.65% in women; 20%, 28 %, and 38%, 0% in men; 6.25%, 12.5%, 25% and, 0% in infants and 33.33%, 46.66% in sheep and 29%, 14, 16% in goats by LA and IHA respectively. ELISA showed highest sensitivity, specificity, and highest accuracy. Hygienic conditions must be applied in case of active toxoplasmosis to minimize transmission of the disease. Women should be routinely tested for Toxoplasma antibodies before and after pregnancy to avoid severe or fatal consequences. https://www.biotech-asia.org/vol10no1/toxoplasmosis-in-women-men-infants-and-animals-in-jazan-district/ Toxoplasma gondii Human Animal Anti-toxoplasma Jazan District.