eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2013-06-28 10 1 10.13005/bbra/1145 10483 article M.N. Abubacker 1 J. Durgadevi 2 C. Sathya 3 K. Leema Rose 4 Department of Biotechnology, National College, Tiruchirappalli - 620 001, India. Department of Biotechnology, National College, Tiruchirappalli - 620 001, India. Department of Botany , Alagappa University, Karaikudi - 630 003, India. Department of Botany, National College, Tiruchirappalli - 620 001, India. An efficient protocol for micropropagation of orchid Dendrobium barbatum Lindl. (Orchidaceae) using the fleshy stem segments was developed. Murashige Skoog (MS) basal medium supplemented with 1.0 mg L of Naphthalene Acetic Acid (NAA) + 2 .0mg L of Benzyl Amino Purine (BAP) for multiple shoot induction. The multiplied shoots were then transferred to half-strength MS medium supplemented with 1 mg L of Indole-3 acetic acid (IAA) or Indole-3 butyric acid (IBA) or NAA. Best response for shoot multiplication was achieved when the medium was supplemented with 1 mg L-1 NAA and 2mg L-1 BAP. While that for rooting with 1 mg l IBA. The plantlets were hardened off and survived plants were released to the shade house then to their epiphytic environment. https://www.biotech-asia.org/vol10no1/micropropagation-of-orchid-dendrobium-barbatum-lindl-orchidaceae-from-fleshy-stem-segments/ Benzyl Amino Purine (BAP); Dendrobium barbatum fleshy stem segment; In vitro