eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2013-06-28 10 1 10.13005/bbra/1121 10344 article B. Jhansi Lakshmi 1 K. Jaganmohanreddy 1 Department of Botany, Kakatiya University, Warangal - 506 009, India. The study has provided a protocol for rapid in vitro propagation by multiple shoot induction of Gardenia sinifera, an important medicinal plant belonging to Rubiaceae family. Multiple shoots were induced in vitro from the nodal segments and immature fruit segments on Murashige and Skoog (MS) medium containing 6-benzylaminopurine (BAP) alone or in combination with naphthalene acetic acid (NAA), IAA, IBA and 2, 4-D ( 2, 4-dichlorophenoxy acetic acid). Internodal segments in combination with BAP (2mg/l) + NAA (0.25 mg/l) observed two shoots and in immature fruit segments maximum number of shoots (4 shoots per explant) was observed on the medium containing BAP (2 mg/l) in combination with NAA (0.5mg/l). Regenerated shoots were rooted on 1/2 MS supplemented with IAA (0.5) proved best rooting from nodal segments. The rooted in vitro raised plantlets were acclimatized in growth chamber and successfully transferred to greenhouse with 80% survival rate. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant. https://www.biotech-asia.org/vol10no1/in-vitro-multiple-shoot-induction-directly-from-different-explants-of-gardenia-resinifera-roth/ Acclimatization; Germplasm conservation; in vitro propagation; Nodal segments