Characterization of Immobilized L-Asparaginase Produced from Pseudomonas aeruginosa 50071 by Solid State Fermentation

Mohsen M. S. Asker¹, Sahera F. Mohamed^1.2* and Osama H. El-sayed²

¹Microbial Biotechnology Department, National Research Center, Dokki, Cairo, Egypt. ²Chemistry Department, College of Science, Jazan University, Jazan, KSA.

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1034

ABSTRACT: The natural polymer sodium alginate is a macropolysaccharide Produced from brown algae has many applications. Anti-leukemia enzyme L-asparaginase (L-ASNase) produced from Pseudomonas aeruginosa 50071 by solid state fermentation was covalently conjugated on sodium alginate. SDS-PAGE showed that the purified L-ASNase was homogeneous with molecular weight of ~ 113 KDa. The immobilized enzyme on 3% sodium alginate showed specific activity of 62 U/mg protein. The immobilized L-ASNase maintained over 90 % of the original activity of free enzyme. Also it showed a slightly thermal stability where the activation energy of denaturation were 28 and 35 Kcal mol-1 for both native and immobilized enzyme, respectively. The Km of sodium alginate immobilized enzyme was about 2 times lower than that of the free enzyme.

KEYWORDS:

Immobilization; L-asparaginase; pseudomonas aeruginosa; solid state fermentation.

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