Manuscript accepted on : July 24, 2007
Published online on: 02-02-2016
F.U. Willie And A. S. Ekop*
Department of Chemistry, University of Uyo, p.m.b 1017, uyo, (Nigeria)
ABSTRACT: An alternative method has been developed for accurate determination of vitamin C in various juice samples using aqueous extract of fresh exoderm of fluted pumpkin seeds. This locally extracted dye (LED) gives a better percentage recovery by titration method when compared to the conventional titrimetric method using 2,6 - Dichlorophenol-indophenol (DCPIP). Student’s t-test statistical analysis for correlated paired data showed that there is no significant difference (a = 0.01, N = 21) in precision and reproducibility in the determination by both the new LED and conventional DCPIP methods. Vitamin C content of 21 samples including natural fruits, commercial fruit juice and vitamin C tablets have been accurately determined using this method. The method developed has also been successfully adapted to spectrophotometric titration for determination of trace amount (<0.064mg/ml) of vitamin C in fruit juice samples.
KEYWORDS: Vitamin C; Determination; Titrimetric method; Local Dye Extract (LED)
Download this article as:
| Copy the following to cite this article: Willie F. U, Ekop A. S. Titrimetric determination of vitamin C in natural and commerical fruit juice samples using a locally extracted dye. Biosci Biotechnol Res Asia 2007;4(2) |
| Copy the following to cite this URL: Weremfo A, Duweijua M, Abassah-Oppong S. Toxicological evaluation of ethanolic root extract of cassia sieberiana in rats. Biosci Biotechnol Res Asia 2007;4(2). Available from: https://www.biotech-asia.org/?p=5881 |
This work is licensed under a Creative Commons Attribution 4.0 International License.
