New Spectrophotometric Methods for the Estimation of Silymarin In Pure and Pharmaceutical Dosage Forms

T. Sathish¹, U. Kiran Babu¹, K. Kishore Kumar¹, N. Pratap², and M. Krishna Prasad¹

¹Center for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam - 530 003 (India). ²Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam - 530 003 (India)

ABSTRACT: Silymarin, as the plant active ingredient of milk thirsle a polyphenolic antioxidant flavanoids, protecting the liver cells membrane and facilitate the recovery of the impaired liver cells. Very few analytical methods have been reported for the determination of Silymarin Estimation of Silymarin with (MBTH & CAS) in pure & pharmaceutical dosage form (Method-I), with fecl3 and 2-2 Bipyrdine (Method – II) by using Elico – uv – Visible- spectrophotometer model SL-150. The concentration range 5-40 mg/ml was obeying beer-lambert’s law, at 520 nm in Method – I and concentration range 5- 25 mg/ml was obeying beer-lambert’s law at 525 nm in Method – II. In case of pharmaceutical dosage forms, the silymarin percent content was found to be 99.50 in both the methods. The proposed methods are applicable for the assay of silymarin in both pure and pharmaceutical dosage forms.

KEYWORDS: Silymarin; MBTH (3-Methyl Benzthia-zolinone-2-Hydrozone)

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