HPTLC Fingerprinting Method to Distinguish Total Extract of Caralluma fimbriata from the Modified Extracts of Caralluma fimbriata

T. Lakshmi*, R. Rajendran, Vijay Raghavan and Antony Silvester

Department of Pharmacology, Saveetha Dental College & Hospitals,Chennai, India. Green Chem Herbal Extracts and Formulations, Bangalore, India.

ABSTRACT: To establish the fingerprint profile of total extract of Caralluma fimbriata using high performance thin layer chromatography (HPTLC) technique. HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software were used. HPTLC finger printing of Caralluma fimbriata leaf extract revealed that the chromatogram obtained with the reference solution shows in the middle part a Dark blue zone [Slimalumaside A] at RF 0.33 and in the upper part a Blue zone [Slimalumaside B] at RF 0.94 was observed. It can be concluded that HPTLC fingerprint analysis of total extract of Caralluma fimbriata can be used as a diagnostic tool for the appropriate identification various extracts.

KEYWORDS: Caralluma adscendens var. fimbriata; HPTLC

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