Volume 15, number 3
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Ameliorating Effects of Badri Cow Urine on Cypermethrin Induced Immunotoxicity and Oxidative Stress in Chicken Lymphocytes Culture System

Sonu Ambwani1, Tanuj Kumar Ambwani2 and Ramswaroop Singh Chauhan3

1Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, GBPUA and T, Pantnagar, Uttarakhand, India.

2Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, GBPUA and T, Pantnagar, Uttarakhand, India.

3Department of Veterinary Pathology, College of Veterinary and Animal Sciences, GBPUA and T, Pantnagar, Uttarakhand, India.

Corresponding Author E-mail: ambwani_sonu@rediffmail.com

DOI : http://dx.doi.org/10.13005/bbra/2679

ABSTRACT: Cow urine has many beneficial properties particularly in the areas of agriculture and therapeutics. It has also been observed during the scientific research that the urine of Indian cows is highly effective against various ailments. Cow urine or ‘gau mutra’ has a unique place in Ayurveda and is suggested for improving general health. Cypermethrin is a widely used composite pyrethroid. It is a broad spectrum, non-cumulative insecticide and a fast-acting neurotoxin. It is reported to exhibit deleterious health impacts on human and/ animal health. Present paper reports ameliorating effects of cow urine distillate (CU) against cypermethrin induced immunotoxicity and oxidative stress in chicken lymphocytes culture employing lymphocyte proliferation assay and nitric oxide (NO) estimation. Cypermethrin treated cells displayed immunotoxic effects as observed by decrease in B and T cells proliferation. In case of combination treatments of cypermethrin and CU, there was increase in B and T cells proliferation as compared to only pesticide treated cells. Nitric oxide estimation revealed enhanced oxidative stress in cypermethrin treated cells in comparison to combination treated groups.

KEYWORDS: Chicken Lymphocytes; Cow Urine; Cypermethrin; Immunotoxicity; Oxidative Stress

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Ambwani S, Ambwani T. K, Chauhan R. S. Ameliorating Effects of Badri Cow Urine on Cypermethrin Induced Immunotoxicity and Oxidative Stress in Chicken Lymphocytes Culture System. Biosci Biotech Res Asia 2018;15(3).

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Ambwani S, Ambwani T. K, Chauhan R. S. Ameliorating Effects of Badri Cow Urine on Cypermethrin Induced Immunotoxicity and Oxidative Stress in Chicken Lymphocytes Culture System. Biosci Biotech Res Asia 2018;15(3). Available from: http://www.biotech-asia.org/?p=31240

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