Antifungal Effect of Flavonoid Extract of Trachyspermum Ammi Plant on the Gene Expression of Pro-Inflammatory Cytokines Such As IL-18 And TNF-Α In Articular THP-1 Monocyte/Macrophages Cells
Maryam Sadat Abtahi1*, Hossein Maghsoudi1, Boshra Hatef2, Parisa Marashi3, Mahnesa Vassaf3
1*Department of Biotechnology Engineering the agricultural orientation, Payame Noor University, po box, 19395-3697, Tehran, IRAN 1Department of Biotechnology, Payame Noor University, po box, 19395-3697, Tehran, IRAN 2Professor assistant of Neuroscience Research Center of Baqiyatallah University of Medical Science, North Artesh Highway, Second Avenue, Tehran, IRAN 3Department of Biotechnology Engineering the agricultural orientation, Payame Noor University, po box, 19395-3697, Tehran, IRAN *Corresponding author E-mail: Abtahi.firstname.lastname@example.org
ABSTRACT: The Trachyspermum ammi plant has anti-fungal properties. The aim of this study is to examine the anti-fungal effects of this plant on the pro-inflammatory bio-markers, which are important in the course of fungal diseases. At first the ethanol extract of the Trachyspermum ammi plant was prepared from the Iranian biological resource center. We used monocyte/macrophage cells as samples because they were similar to the cells with fungal infection. Then THP-1 monocyte/macrophages (5×105 cells/well) were incubated at a humidity of 90%, 5% CO2, 37˚C for 72 h with control media alone or Trachyspermum ammi at concentrations of 10 μg/ml. One set of cells was activated for 1 h with mannose (3 mg/ml) for both reverse-transcriptase Polymerase chain reaction and real-time Polymerase chain reaction analysis of Tumor necrosis factor alpha (TNF-α), Interleukin-18 (IL-18) expression. the activated cells by mannose with a plant extract redused the Interleukin-18 and Tumor necrosis factor alpha expression as activated cell by mannose with fluconazole (p-value=0.91 for IL-18, p-valu=0.25 for TNF-α). Based on the PCR and realtime methode to analysis of gene expression, Trachyspermum ammi plant such as the flavonoids has noticeable anti-fungal properties.
KEYWORDS: Trachyspermum ammi; THP-1 Monocyte/Macrophage; Fungal diseasesDownload this article as:
|Copy the following to cite this article:
Abtahi M. S, Maghsoudi H, Hatef B, Marashi P, Vassaf M. Antifungal Effect of Flavonoid Extract of Trachyspermum Ammi Plant on the Gene Expression of Pro-Inflammatory Cytokines Such As IL-18 And TNF-Α In Articular THP-1 Monocyte/Macrophages Cells. Biosci Biotech Res Asia 2015;12(2)